Bioconjugate Techniques
暫譯: 生物偶聯技術
Greg T. Hermanson
- 出版商: Academic Press
- 出版日期: 1996-01-22
- 售價: $3,840
- 貴賓價: 9.5 折 $3,648
- 語言: 英文
- 頁數: 785
- 裝訂: Paperback
- ISBN: 0123423368
- ISBN-13: 9780123423368
-
相關分類:
生物資訊 Bioinformatics
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商品描述
Description
Bioconjugate Techniques is the essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Armed with this information and the abundant protocols provided, readers will form unique complexes that can be used for detecting, quantifying, and targeting important analytes. This book helps readers make: high activity antibody-enzymes conjugates, immunotoxins, immunogen complexes, liposome conjugates; as well as biotinylated molecules, avidin or streptavidin conjugates, colloidal gold labeled proteins, PEG or dextran complexes, labeled oligonucleotide probes, and fluorescently tagged or radiolabeled molecules.
商品描述(中文翻譯)
**描述**
《生物偶聯技術》(Bioconjugate Techniques)是修改和交聯生物分子的基本指南,適用於研究、診斷和治療。它提供了有關化學、試劑系統和創建標記或偶聯分子的實用應用的詳細信息。書中描述了數十種反應,並詳細介紹了數百種商業可用的試劑,以及這些試劑在修改或交聯肽和蛋白質、糖和多醣、核酸和寡核苷酸、脂質和合成聚合物中的使用。讀者掌握這些信息和提供的豐富實驗步驟後,將能形成獨特的複合物,這些複合物可用於檢測、定量和靶向重要的分析物。本書幫助讀者製作:高活性抗體-酶偶聯物、免疫毒素、免疫原複合物、脂質體偶聯物;以及生物素化分子、親和素或鏈親和素偶聯物、膠體金標記的蛋白質、PEG或右旋糖酐複合物、標記的寡核苷酸探針,以及帶有螢光標記或放射性標記的分子。