Total Internal Reflection Flurescence: Total Internal Reflection Excitation and Near Field Emissionoptical Evanescence Microscopy (Tirk)

This book offers a complete presentation of the physics, math, and experimental setups for both TIRF and related evanescence microscopies. It covers evanescence in both fluorescence excitation or emission. It also discusses, in detail, the theory, setups, and practical biological/biochemical applications for combinations of evanescence microscopies with other optical techniques such as polarization, photobleaching, correlation spectroscopy, scattering, image enhancement, optical force, two-photon, energy transfer, structured illumination, scanning, quenching, and single molecule detection. Physical and qualitative discussions augment the rigorous math, making the book accessible and interesting to a wide range of audiences with backgrounds from biology to chemistry to physics. The book also contains useful step-by-step guides to building, modifying, and aligning TIRF microscopy systems for specialized purposes.

Key Features:

• Suitable for both basic and advanced levels
• Offers combinations with related techniques
• Relationship of evanescent effects between excitation and emission explained
• Practical guide to the optics as well

本書完整介紹了TIRF和相關消逝顯微術的物理、數學和實驗設置。它涵蓋了螢光激發或發射中的消逝現象。詳細討論了消逝顯微術與極化、光漂白、相關光譜學、散射、圖像增強、光力、雙光子、能量轉移、結構照明、掃描、熄滅和單分子檢測等其他光學技術的結合理論、設置和實際生物/生化應用。物理和定性討論增強了嚴謹的數學，使本書對從生物學到化學到物理等各種背景的讀者具有可讀性和趣味性。本書還包含有用的逐步指南，以建立、修改和校準專門用途的TIRF顯微術系統。

主要特點：
- 適用於基礎和高級水平
- 提供與相關技術的結合
- 解釋了激發和發射之間的消逝效應關係
- 同時提供實用的光學指南