Stem Cell Renewal and Cell-Cell Communication: Methods and Protocols

1. Inference of Ligand-Receptor Pairs from Single-Cell Transcriptomics Data

Mirjana Efremova and Roser Vento-Tormo

2. Multiple Imaging Modalities for Cell-Cell Communication via Calcium Mobilizations in Corneal Epithelial Cells

Yoonjoo K. Lee, Kristen L. Segars, and Vickery Trinkaus-Randall

3. Interactions of Hematopoietic Stem Cells with Bone Marrow Niche

Xinghui Zhao, Cuiping Zhang, Xiaojing Cui, and Ying Liang

4. Ex Vivo Modeling of Hematopoietic Stem Cell Homing to the Fetal Liver

Amina Mohammadalipour, Miguel F. Diaz, Sumedha Pareek, and Pamela L. Wenzel

5. Analysis of Epithelial Architecture and Planar Spindle Orientation in the Drosophila Wing Disc

Yu-ichiro Nakajima

6. A Co-Culture Model to Study the Effect of Kidney Fibroblast-p90RSK on Epithelial Cell Survival

Ling Lin, Samantha White, and Kebin Hu

7. Calcium Fluorescence Recordings from Neuroepithelial Stem Cells

Masayuki Yamashita

8. Ultrastructural Analysis of Cell-Cell Interactions in Drosophila Ovary

Matthew Antel, Valentina Baena, Mark Terasaki, and Mayu Inaba

9. TIRF Microscopy as a Tool to Determine Exosome Composition

Noa B. Martín-Cófreces, Daniel Torralba, Marta Lozano-Prieto, Nieves Fernández-Gallego, and Francisco Sánchez-Madrid

10. Rapid Visualization of Intracellular Vesicle Events during Synaptic Stimulation

Noa B. Martín-Cófreces, Amelia Rojas-Gomez, Sara G. Dosil, Irene Fernandez-Delgado, and Francisco Sánchez-Madrid

11. Monitoring of Active Notch Signaling in Mouse Bladder Urothelium

Panagiotis Karakaidos and Theodoros Rampias

12. Examining Local Cell-to-Cell Signaling in the Kidney Using ATP Biosensing

Gareth W. Price, Joe A. Potter, Bethany M. Williams, Chelsy L. Cliff, Mark J. Wall, Claire E. Hills, and Paul E. Squires

13. Isolation and Assessment of Pancreatic Islets Versus Dispersed Beta Cells: A Straightforward Approach to Examine Cell-Cell Communication

Rachel T. Scarl, William J. Koch, Kathryn L. Corbin, and Craig S. Nunemaker

14. Promoter Pull-Down Assay: A Biochemical Screen for DNA-Binding Proteins

Ryan R. Chaparian and Julia C. van Kessel

15. Purification of the Vibrio Quorum-Sensing Transcription Factors LuxR, HapR, and SmcR

Jane D. Newman and Julia C. van Kessel

16. Preserving Cytonemes for Immunocytochemistry of Cultured Adherent Cells

Sally Rogers and Steffen Scholpp