Cell Viability Assays: Methods and Protocols
暫譯: 細胞存活率測定:方法與實驗步驟

Friedrich, Oliver, Gilbert, Daniel F.

  • 出版商: Humana Press
  • 出版日期: 2024-05-18
  • 售價: $6,800
  • 貴賓價: 9.5$6,460
  • 語言: 英文
  • 頁數: 502
  • 裝訂: Quality Paper - also called trade paper
  • ISBN: 1071630547
  • ISBN-13: 9781071630549
  • 相關分類: 生物資訊 Bioinformatics
  • 無法訂購

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商品描述

This updated edition explores assessing cell viability as a measure for cell fitness under conditions of physiological and patho-physiological stress as well as challenging conditions to cellular and tissue homeostasis, and accounts for the ongoing 2D-to-3D development with topics and assays that target cell viability, mobility, and functionality of tissues and organs, natural or bioartificial, in 3D. The book's contents span a wide range of viability and functionality assays, from impedance spectroscopy to chemiluminescence, fluorescence and label-free optical detection methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Cell Viability Assays: Methods and Protocols, Second Edition serves as a valuable resource to the growing community in bioinspired life sciences, biomedical sciences, and biotechnology by providing more standardized protocols to probe the "wellbeing" of cells in various environments.

商品描述(中文翻譯)

本更新版探討了評估細胞活性作為細胞適應性的一種指標,特別是在生理和病理生理壓力條件下,以及對細胞和組織穩態的挑戰條件下,並考慮到持續的2D到3D發展,涵蓋針對細胞活性、移動性和組織及器官(無論是自然的還是生物人工的)功能性的主題和測試,均在3D環境中進行。本書內容涵蓋了廣泛的活性和功能性測試,從阻抗光譜學到化學發光、螢光及無標記光學檢測方法。作為成功的《分子生物學方法》(Methods in Molecular Biology)系列的一部分,各章節包括對其各自主題的介紹、所需材料和試劑的清單、逐步的可重複實驗室操作流程,以及故障排除和避免已知陷阱的提示。
《細胞活性測試:方法與協議,第二版》是一本權威且最新的資源,為日益增長的生物啟發生命科學、生物醫學科學和生物技術社群提供了更標準化的協議,以探測細胞在各種環境中的「健康狀態」。

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