Proteomics for Drug Discovery
Blonder, Josip
- 出版商: Humana Press
- 出版日期: 2024-07-26
- 售價: $6,540
- 貴賓價: 9.5 折 $6,213
- 語言: 英文
- 頁數: 371
- 裝訂: Hardcover - also called cloth, retail trade, or trade
- ISBN: 1071639218
- ISBN-13: 9781071639214
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商品描述
This detailed volume explores a wide range of experimental and bioinformatic proteomics methods applicable to the drug discovery process. Focused on drug target discovery, validation, characterization, and quantitation, the book delves into bottom-up data-dependent (DDA) shotgun and data-independent acquisition (DIA) proteomics, cell surface proteomics, proteogenomics, chemoproteomics, methodologies focused on posttranslational modifications, single cell proteomics, 3D cell culture proteomics, organoids, and laser capture microdissection (LCM) assisted tissue proteogenomics. Advanced quantitative techniques, which include label-free, mass tagging, and targeted immuno-MRM proteomics, along with top-down proteomics for validation and characterization of selected protein targets, are also described. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding foreseeable pitfalls.
Authoritative and practical, Proteomics for Drug Discovery serves as an ideal guide for researchers striving to push the field of drug discovery via MS-based proteomics to new frontiers.
商品描述(中文翻譯)
這本詳細的書籍探討了廣泛的實驗和生物信息學蛋白質組學方法,適用於藥物發現過程。該書專注於藥物靶點的發現、驗證、表徵和定量,深入探討了自下而上的數據依賴(DDA)shotgun和數據獨立獲取(DIA)蛋白質組學、細胞表面蛋白質組學、蛋白質組學、化學蛋白質組學、專注於翻譯後修飾的方法、單個細胞蛋白質組學、三維細胞培養蛋白質組學、器官樣品和激光捕獲微切片(LCM)輔助組織蛋白質組學。還描述了先進的定量技術,包括無標籤、質量標記和靶向免疫-MRM蛋白質組學,以及用於驗證和表徵選定蛋白質靶點的自上而下蛋白質組學。本書是為極為成功的《分子生物學方法》系列撰寫的,章節包括對各自主題的介紹、所需材料和試劑的列表、逐步可重複的實驗室協議,以及疑難解答和避免可預見問題的提示。
權威且實用,《藥物發現的蛋白質組學》是研究人員努力通過基於質譜儀的蛋白質組學將藥物發現領域推向新的前沿的理想指南。