Plant Biotic Stress: Methods and Protocols
暫譯: 植物生物性壓力:方法與實驗程序

Senthil-Kumar, Muthappa, Ramanna, Hema

  • 出版商: Humana Press
  • 出版日期: 2025-10-02
  • 售價: $7,970
  • 貴賓價: 9.5$7,572
  • 語言: 英文
  • 頁數: 317
  • 裝訂: Hardcover - also called cloth, retail trade, or trade
  • ISBN: 1071647458
  • ISBN-13: 9781071647455
  • 相關分類: 生物資訊 Bioinformatics
  • 無法訂購

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商品描述

This volume presents a comprehensive collection of protocols and techniques commonly used in research on plant-biotic interactions. Chapters guide readers through general plant-pathogen interactions, RNA interference (RNAi) in Okra, spot inoculation of begomoviruses, natural transmission, RNA viruses, enzyme-linked immunosorbent, reverse transcription polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (RT-LAMP), northern blot hybridization, and next-generation sequencing (NGS) for in-depth analysis of viral populations and mutation patterns. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Biotic Stress: Methods and Protocols aims to be comprehensive guide for researchers in the field.

商品描述(中文翻譯)

本卷提供了一個全面的協議和技術集合,這些協議和技術通常用於植物與生物相互作用的研究。各章節引導讀者了解一般的植物-病原體相互作用、在秋葵(Okra)中的RNA干擾(RNAi)、對begomoviruses的點接種、自然傳播、RNA病毒、酶聯免疫吸附、反轉錄聚合酶鏈反應(RT-PCR)、環介導等溫擴增(RT-LAMP)、北方印跡雜交以及下一代測序(NGS),以深入分析病毒群體和突變模式。這些章節以成功的《分子生物學方法(Methods in Molecular Biology)》系列格式撰寫,包括對材料的簡要介紹、所需材料和試劑的清單、逐步的、易於重複的實驗室協議,以及一個註釋部分,重點介紹故障排除和避免已知陷阱的提示。《植物生物壓力:方法與協議(Plant Biotic Stress: Methods and Protocols)》權威且前沿,旨在成為該領域研究人員的全面指南。

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