Yeast Systems Biology: Methods and Protocols
暫譯: 酵母系統生物學:方法與協議

Oliver, Stephen G., Castrillo, Juan I.

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商品描述

This second edition volume expands on the previous edition with a look at the latest advances in techniques to study yeast and its core set of interactions, modules, architectures, and network dynamics that are common in all eukaryotes. The chapters in this book are organized into Four Parts: Part One provides readers with an update on the development of novel experimental and computational approaches to yeast systems biology; Part Two explores high-throughput methods used to study yeast epigenome, transcriptome, proteome, and metabolome; Part Three talks about computational systems biology, and focuses on data management, dynamic modeling, constraint-based models of metabolic networks, and multi-level 'omics data; while Part Four looks at experimental platforms that utilize yeast to model systemic human diseases such as Alzheimer's and Parkinson's diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics; lists of the necessary materials and reagents; step-by-step, readily reproducible laboratory protocols; and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Yeast System Biology: Methods and Protocols, Second Edition is a valuable tool for graduate students, post-doctoral researchers, and experts who are interested in learning about the latest developments in the study of yeast.


商品描述(中文翻譯)

這本第二版的書籍擴展了前一版的內容,探討了研究酵母的最新技術進展,以及其核心互動、模組、架構和在所有真核生物中普遍存在的網絡動態。本書的章節分為四個部分:第一部分為讀者提供有關酵母系統生物學的新實驗和計算方法的最新進展;第二部分探討用於研究酵母表觀基因組、轉錄組、蛋白質組和代謝組的高通量方法;第三部分談論計算系統生物學,重點在於數據管理、動態建模、基於約束的代謝網絡模型以及多層次的“組學”數據;而第四部分則關注利用酵母來模擬系統性人類疾病(如阿茲海默症和帕金森病)的實驗平台。這本書採用成功的《分子生物學方法》(Methods in Molecular Biology)系列格式編寫,章節包括各自主題的介紹;所需材料和試劑的清單;逐步、易於重複的實驗室操作流程;以及故障排除和避免已知陷阱的提示。

前沿且全面的《酵母系統生物學:方法與協議,第二版》(Yeast System Biology: Methods and Protocols, Second Edition)是研究生、博士後研究人員和對酵母研究最新發展感興趣的專家的寶貴工具。