Carbonic Anhydrase as Drug Target: Thermodynamics and Structure of Inhibitor Binding

Matulis, Daumantas

  • 出版商: Springer
  • 出版日期: 2019-06-03
  • 售價: $5,940
  • 貴賓價: 9.5$5,643
  • 語言: 英文
  • 頁數: 353
  • 裝訂: Hardcover - also called cloth, retail trade, or trade
  • ISBN: 3030127788
  • ISBN-13: 9783030127787
  • 相關分類: 熱力學 Thermodynamics
  • 下單後立即進貨 (約1週~2週)

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商品描述

This book offers deep insights into the thermodynamics and molecular structures of the twelve catalytically active isoforms of human carbonic anhydrase (CA) with a particular focus on inhibitor binding for drug design. X-ray crystallographic structures in combination with enzyme kinetic testing provide information on the interaction of CAs and their inhibitors, knowledge which is crucial for rational drug design. CAs are zinc carrying enzymes that catalyse the reversible interconversion of carbon dioxide and bicarbonate and are involved in numerous cellular processes. They are therefore a common target for drugs. The suppression of CA activities through inhibitory compounds has found application for example in diuretics and in glaucoma therapy. In this book methods used to determine binding thermodynamics of inhibitory compounds (Isothermal titration calorimetry, Fluorescent thermal shift assay/differential scanning fluorimetry and others) will be compared in detail. Also types and chemical synthesis of CA inhibitors, the use of antibodies against CAs as well as inhibitor application in animals are discussed.

作者簡介

Prof. Dr. Daumantas Matulis

Vilnius University

Life Sciences Center

Institute of Biotechnology

Department of Biothermodynamics and Drug Design

Sauletekio 7, 10257 Vilnius

Lithuania