Classical and Molecular Plant Cytogenetics: A Laboratory Manual
暫譯: 經典與分子植物細胞遺傳學：實驗室手冊
Jha, Timir Baran, Halder, Mihir
- 出版商: Springer
- 出版日期: 2026-05-26
- 售價: $4,440
- 貴賓價: 9.5 折 $4,218
- 語言: 英文
- 頁數: 326
- 裝訂: Hardcover - also called cloth, retail trade, or trade
- ISBN: 9819582644
- ISBN-13: 9789819582648
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相關分類:
生物資訊 Bioinformatics
海外代購書籍(需單獨結帳)
商品描述
This laboratory manual offers a hands-on approach to plant chromosome analysis, combining classical cytogenetics with modern molecular techniques. It begins with an introduction to cell division and plant chromosome structure, and follows into fundamental microscopy skills and the preparation of somatic and gametic chromosomes using both classical and enzymatic maceration methods. Detailed staining protocols, karyomorphological analysis and photomicrography techniques are covered to ensure reproducibility and clarity in chromosome visualization. This volume further includes real-world case studies using widely studied plant species such as Allium cepa, Nigella sativa, and Aloe vera, offering valuable comparative insight into chromosomal structure, number and anomalies. The chapters cover advanced topics including fluorescent in situ hybridization (FISH), genome size estimation and the C-value paradox, along with a primer on flow cytometry and biostatistical analysis for chromosome data.
Supported with illustrations, protocols, and a glossary of instruments, this book serves as an essential reference for cytogenetics laboratories and teaching institutions. Designed for students, researchers, and laboratory professionals in plant biology, cytogenetics and molecular sciences, this book provides a clear and practical understanding of plant chromosome behavior and visualization.
商品描述(中文翻譯)
這本實驗手冊提供了一種實作導向的植物染色體分析方法，結合了傳統細胞遺傳學與現代分子技術。手冊首先介紹了細胞分裂和植物染色體結構，接著進入基本的顯微鏡技能，以及使用傳統和酶解法準備體細胞和配子染色體。詳細的染色協議、核型形態分析和顯微攝影技術被涵蓋，以確保染色體可視化的重現性和清晰度。本書還包括使用廣泛研究的植物物種如 Allium cepa、Nigella sativa 和 Aloe vera 的實際案例研究，提供有價值的比較見解，探討染色體的結構、數量和異常。各章節涵蓋了進階主題，包括螢光原位雜交（FISH）、基因組大小估算和C值悖論，以及流式細胞術和染色體數據的生物統計分析入門。
本書配有插圖、協議和儀器詞彙表，作為細胞遺傳學實驗室和教學機構的重要參考資料。專為植物生物學、細胞遺傳學和分子科學的學生、研究人員和實驗室專業人員設計，本書提供了對植物染色體行為和可視化的清晰且實用的理解。
作者簡介
Dr. Timir Baran Jha is a retired faculty member of the West Bengal Senior Education Service (WBSES), India. He joined the West Bengal Education Service (WBES) in 1979 and has extensive experience teaching both undergraduate and postgraduate courses at various government colleges in West Bengal, India, focusing on plant biotechnology and plant cytogenetics. For over four decades, Dr. Jha has conducted research in the fields of plant biotechnology and plant chromosomes. He has authored a book on plant biotechnology and published more than 90 research papers in national and international journals in his areas of expertise. Although retired, Dr. Jha remains active in teaching and research. He is currently working on developing a chromosomal database for valuable Indian crop plants using molecular cytogenetics.
Dr. Mihir Halder is currently working as an assistant professor in the Post-Graduate Department of Botany at Barasat Government College in West Bengal, India. He earned his bachelor's degree in botany in 2007 from Presidency College, Kolkata, and his master's degree in botany in 2009 from the University of Calcutta. In 2017, he received his Ph.D. in biochemistry from the University of Calcutta, India. With over 10 years of experience, Dr. Halder has been actively teaching courses in genetics, cell and molecular biology, and plant biotechnology. He has made significant research contributions in the fields of plant cytogenetics and plant biotechnology, having published 23 research papers and book chapters.
作者簡介(中文翻譯)
蒂米爾·巴蘭·賈（Dr. Timir Baran Jha）是印度西孟加拉州高級教育服務（WBSES）的退休教職員。他於1979年加入西孟加拉教育服務（WBES），在印度西孟加拉的多所政府大學教授本科和研究生課程，專注於植物生物技術和植物細胞遺傳學。四十多年來，賈博士在植物生物技術和植物染色體領域進行研究。他著有一本植物生物技術的書籍，並在其專業領域內的國內外期刊上發表了90多篇研究論文。儘管已退休，賈博士仍然活躍於教學和研究。目前，他正在利用分子細胞遺傳學開發一個有價值的印度作物植物的染色體數據庫。
米希爾·哈爾德（Dr. Mihir Halder）目前在印度西孟加拉的巴拉薩特政府學院擔任植物學研究所的助理教授。他於2007年在加爾各答的總統學院獲得植物學學士學位，並於2009年在加爾各答大學獲得植物學碩士學位。2017年，他在印度加爾各答大學獲得生物化學博士學位。哈爾德博士擁有超過10年的教學經驗，積極教授遺傳學、細胞與分子生物學以及植物生物技術等課程。他在植物細胞遺傳學和植物生物技術領域做出了重要的研究貢獻，發表了23篇研究論文和書籍章節。
