Arabidopsis Protocols

Part I: Arabidopsis Resources

1. Handling Arabidopsis and other Brassicaceae: Growth, Preservation of Seeds, Transformation and Genetic Crosses

Christopher Calhoun, Deborah Crist, Emma Knee, Julie Miller, Eva Nagy, and David Somers

2. Bioinformatic Tools in Arabidopsis Research

G. Alex Mason, Alex Cantó-Pastor, Siobhan M. Brady, and Nicholas J. Provart

Part II: Genetic Techniques in Arabidopsis

3. Analyses of Natural Variation: Field Experiments and Nucleotide Diversity for Your Favorite Gene

Carlos Alonso-Blanco, Belén Méndez-Vigo, and F. Xavier Picó

4. Non-sterile Grafting Methods for Arabidopsis

Nien-Chen Huang, and Tien-Shin Yu

5. CRISPR/Cas9-based Genome Editing Toolbox for Arabidopsis thaliana

Daisuke Miki, Gaurav Zinta, Fangnan Peng, Zhengyan Feng, and Jian-Kang Zhu

6. Transient Transformation of A. thaliana Seedlings by Vacuum Infiltration

César Bernat-Silvestre, Vanessa Vieira, Fernando Aniento, and Ma Jesús Marcote

7. Transient Expression of Fluorescent Fusion Proteins in Arabidopsis Protoplasts

Yonglun Zeng, Changyang Ji, Youshun Lin, and Liwen Jiang

8. Photosynthetic and Heterotrophic Cell Suspension Cultures

Enrico Cortese, Luca Carraretto, Barbara Baldan, and Lorella Navazio

9. Genome-wide Association Studies in Arabidopsis thaliana: Statistical Analysis and Network-Based Augmentation of Signals

Tak Lee and Insuk Lee

Part III: Molecular and Cell biological Techniques in Arabidopsis

10. Visualising and Measuring Single Locus Dynamics in Arabidopsis thaliana

Anis Meschichi and Stefanie Rosa

11. Identification and Quantification of Small RNAs

Di Sun, Zeyang Ma, Jiaying Zhu, and Xiuren Zhang

12. Flow Cytometry and Sorting in Arabidopsis

David W. Galbraith and Guiling Sun

13. Live Imaging of Arabidopsis Leaf and Vegetative Meristem Development

Monica Pia Caggiano, Xiulian Yu, Carolyn Ohno, Pia Sappl, and Marcus G. Heisler

14. Using Genetically Encoded Fluorescent Biosensors for Quantitative in vivo Imaging

Akira Yoshinari, Jacob Moe-Lange, Thomas J. Kleist, Heather N. Cartwright, David A. Quint, David W. Ehrhardt, Wolf B. Frommer, and Masayoshi Nakamura

15. Using Tripartite Split-sfGFP for the Study of Membrane Protein-Protein Interactions

Tzu-Yin Liu

16. High-pressure Freezing and Freeze Substitution for Transmission Electron Microscopy Imaging and Immunogold-labeling

Marisa S. Otegui

17. Atomic Force Microscopy to Study Cell Wall Mechanics in Plants

Mateusz Majda

18. Identification and Characterization of Reproductive Mutations in Arabidopsis

María Flores-Tornero, Stefanie Sprunck, and Thomas Dresselhaus

Part IV: Biochemical and Physiological Techniques in Arabidopsis

19. Metabolomic Analysis of Natural Variation in Arabidopsis

Si Wu, Saleh Alseekh, Yariv Brotman, and Alisdair R. Fernie

20. Untargeted Metabolomics of Arabidopsis Stomatal Immunity

Lisa David, Jianing Kang, and Sixue Chen

21. Biotin-based Proximity Labeling Of Protein Complexes in Planta

Madiha Khan, Rajagopal Subramaniam, and Darrell Desveaux

22. Phosphoproteomic Analysis of Plant Membranes

Lin Xi, Waltr