Fundamentals of Fluorescence Microscopy: Exploring Life with Light
暫譯: 螢光顯微鏡基礎：用光探索生命

Mondal, Partha Pratim, Diaspro, Alberto

Fundamentals of Fluorescence Microscopy: Exploring Life with Light
  • 出版商: Springer
  • 出版日期: 2016-08-23
  • 售價: $2,510
  • 貴賓價: 9.5 折 $2,385
  • 語言: 英文
  • 頁數: 218
  • 裝訂: Quality Paper - also called trade paper
  • ISBN: 940177966X
  • ISBN-13: 9789401779661
  • 相關分類: 光學 Optics、生物資訊 Bioinformatics

    • 海外代購書籍(需單獨結帳)

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商品描述

This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors.
The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices.
The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.

商品描述(中文翻譯)

本書從入門級別開始，帶領讀者深入了解熒光成像和超解析技術的最先進主題，這些技術促成了納米生物成像、多光子顯微鏡、納米計量學和納米感測器等新發展。熒光顯微鏡和成像這一跨學科主題需要對成像光學和分子物理有全面的了解。因此，本書在深入探討先進成像系統及其應用之前，首先介紹光學成像的概念。此外，分子軌道理論是呈現分子物理的重要基礎，有助於全面理解光與物質在幾何焦點的相互作用。這兩個學科之間有一些重疊，因為光控制分子的分子狀態，反過來，分子狀態又控制所發射的光。這兩種機制共同決定了基本的成像因素，例如分子橫截面、斯托克斯位移、發射和吸收光譜、量子產率、信號對噪聲比、福斯特共振能量轉移（FRET）、光漂白後的熒光恢復（FRAP）和熒光壽命。這些因素構成了許多基於熒光的設備的基礎。本書分為兩個部分。第一部分處理成像光學的基本知識及其應用。進階部分則關注過去十年中發展的幾種成像技術及相關儀器，指向遠場衍射無限成像。

作者簡介

Alberto Diaspro, born in Genoa, Italy, on April 7, 1959, received his Laurea in Electronic Engineering in 1983, Univ.of Genoa. Dir. Nanophys at Italian Institute of Technology (IIT), Deputy Director of IIT, Prof. Appl. Phys. at Univ.of Genoa. President of Optics Within Life Sciences, IEEE Senior member and Editor in chief of Microscopy Research and Technique (7/2013).
Alberto Diaspro is a member of the Bioengineering and Robotics PhD Advisory Committee (University of Genova) and affiliated with the Institute of Biophysics of the National Research Council (CNR). He founded LAMBS in 2003 (Laboratory for Advanced Microscopy, Bioimaging and Spectroscopy, www.lambs.it). President of EBSA (European Biophysical Societies' Association, www.ebsa.org) (2009?2011), President of OWLS (Optics with Life Sciences) (2010?2014) and member of the International Relations Committee of the Biophysical Society. ISI Pubs > 250, H=30, cit > 4000 (Google Scholar). AD is involved in research development of instrumentation for applications in biophysics and biomedical engineering and in nanobiotechnological projects.

Partha Pratim Mondal is an assistant professor at the Indian Institute of Science in the department of Instrumentation and Applied Physics. He received his Ph.D. in Physics from the Indian Institute of Science, Bangalore, India in 2005. He has been a researcher at the MIT, Cambridge, MA, USA and at the universities of Genova, Italy and also was a ICTP Fellow, Trieste, Italy. He has more than 30 journal publications to his name and 18 contributions to conference proceedings. Currently, he is Associate Editor for Scientific Reports (Nature Publishing), Academic Editor to the AIP Advances journal and an editorial board member to 'Microscopy, Research and Technique (Wiley).

作者簡介(中文翻譯)

阿爾貝托·迪亞斯普羅（Alberto Diaspro）於1959年4月7日出生於意大利熱那亞，於1983年獲得熱那亞大學電子工程學位。他是意大利科技研究院（IIT）納米物理學主任，IIT副主任，並擔任熱那亞大學應用物理學教授。他是生命科學光學協會的會長，IEEE資深會員及《顯微鏡研究與技術》（Microscopy Research and Technique）的主編（2013年7月）。阿爾貝托·迪亞斯普羅是熱那亞大學生物工程與機器人博士諮詢委員會的成員，並與國家研究委員會（CNR）生物物理學研究所有關聯。他於2003年創立了LAMBS（先進顯微鏡、生物影像和光譜學實驗室，www.lambs.it）。他曾擔任歐洲生物物理學會聯合會（EBSA，www.ebsa.org）會長（2009-2011），生命科學光學協會（OWLS）會長（2010-2014），並且是生物物理學會國際關係委員會的成員。ISI出版物超過250篇，H指數為30，引用次數超過4000（Google Scholar）。阿爾貝托·迪亞斯普羅參與生物物理學和生物醫學工程應用儀器的研究開發，以及納米生物技術項目。

帕爾塔·普拉蒂姆·蒙達爾（Partha Pratim Mondal）是印度科學研究院儀器與應用物理系的助理教授。他於2005年在印度班加羅爾的印度科學研究院獲得物理學博士學位。他曾在美國麻省劍橋的麻省理工學院（MIT）及意大利熱那亞大學擔任研究員，並曾是意大利的ICTP研究員。他擁有超過30篇期刊出版物及18篇會議論文。目前，他是《科學報告》（Scientific Reports，Nature Publishing）的副編輯，AIP Advances期刊的學術編輯，以及《顯微鏡研究與技術》（Microscopy, Research and Technique，Wiley）的編輯委員會成員。

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