In Situ Hybridization in Electron Microscopy
暫譯: 電子顯微鏡中的原位雜交技術

Morel, Gerard, Cavalier, Annie, Williams, Lynda

  • 出版商: CRC
  • 出版日期: 2020-12-18
  • 售價: $2,760
  • 貴賓價: 9.5$2,622
  • 語言: 英文
  • 頁數: 472
  • 裝訂: Quality Paper - also called trade paper
  • ISBN: 0367455374
  • ISBN-13: 9780367455378
  • 相關分類: 生物資訊 Bioinformatics理工類
  • 海外代購書籍(需單獨結帳)

相關主題

商品描述

In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization of genes:
hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method)
hybridization prior to embedding (pre-embedding)
hybridization on ultrathin sections of frozen tissue (frozen tissue method).
For each technique, the different stages are described in detail: the preparation of tissue, pretreatment, hybridization, and visualization of the hybridization products.

The book combines theory and practice, starting with the basic principles, then breaking down the experimental process into successive steps illustrated by numerous diagrams, detailed protocols, and tables. This is all done in a format that uses parallel columns to convey useful comments next to the theory and practical details alongside each stage of the protocol. Additionally, the summary tables provide the criteria for choosing the probe type and technique, and a detailed index aids in the search for information. in situ Hybridization In Electron Microscopy is an essential companion for applying these methods at the electron microscopic level.

商品描述(中文翻譯)

在位點雜交(in situ hybridization)是一種技術,允許在單個細胞中可視化特定的 DNA 和 RNA 序列,這對於研究異質細胞群體中的核酸特別重要。《在位點雜交與電子顯微鏡》回顧了三種主要方法,用於基因的超微結構可視化:
嵌入在親水性樹脂中的組織超薄切片上的雜交(後嵌入法)
嵌入前的雜交(前嵌入法)
冷凍組織的超薄切片上的雜交(冷凍組織法)。
對於每種技術,詳細描述了不同的階段:組織的準備、預處理、雜交和雜交產物的可視化。

本書結合了理論與實踐,從基本原則開始,然後將實驗過程分解為連續的步驟,並用大量圖示、詳細的實驗流程和表格進行說明。所有內容均以平行列的格式呈現,便於在理論和實踐細節旁邊傳達有用的註解。此外,摘要表提供了選擇探針類型和技術的標準,詳細的索引有助於信息的查找。《在位點雜交與電子顯微鏡》是應用這些方法於電子顯微鏡層面的必備伴侶。

作者簡介

Morel, Gerard - Cavalier, Annie - Williams, Lynda

作者簡介(中文翻譯)

莫雷爾,傑拉德 - 卡瓦利耶,安妮 - 威廉斯,琳達

最後瀏覽商品 (20)