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商品描述
This book reviews the development of cell-free production platforms and offers an authoritative perspective of the latest advances and methodologies in cell-free production systems. Readers will discover the biomanufacturing potential of in vitro biotransformation (ivBT) employing purified cascade multi-enzymes, the development of hydrogel-based multi-enzymatic systems for biosynthesis, and novel insights into the optimization of biocatalytic processes. Additionally, the book explores the cell-free production and regeneration of cofactors, shedding light on strategies to enhance the efficiency and sustainability of cellular processes.
In this book, particular attention is given to the progress of cell-free in vitro evolution techniques for optimizing enzyme performance, and the book also presents the integration of rapid and finely-tuned expression systems for deployable sensing applications, revolutionizing the field of biosensing. The synthesis and electrophysiological analysis of multipass voltage-gated ion channels tethered in microsomal membranes are explored, providing a deep understanding of cellular function at the molecular level. Lastly, the book covers compartmentalized cell-free expression systems for building synthetic cells, showcasing the potential for constructing artificial cellular systems with unique functionalities. Given its breadth, this book appeals to academics, researchers, and professionals interested in the forefront of biotechnology, and together with the companion volume "Cell-free Macromolecular Synthesis", both books highlight the research progresses on the basic and applied research of cell-free production systems in the last few years, being invaluable resources in the field.Chapter "Cell-free synthesis and electrophysiological analysis of multipass voltage-gated ion channels tethered in microsomal membranes" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
商品描述(中文翻譯)
本書回顧了無細胞生產平台的發展，並提供了對無細胞生產系統最新進展和方法論的權威觀點。讀者將發現利用純化的級聯多酶進行的體外生物轉化（in vitro biotransformation, ivBT）的生物製造潛力，水凝膠基多酶系統在生物合成中的發展，以及對生物催化過程優化的新見解。此外，本書探討了無細胞生產和輔因子的再生，闡明了提高細胞過程效率和可持續性的策略。
本書特別關注無細胞體外進化技術在優化酶性能方面的進展，並介紹了快速且精細調整的表達系統在可部署感測應用中的整合，徹底改變了生物感測領域。書中探討了固定在微粒膜中的多通道電壓門控離子通道的合成和電生理分析，提供了對分子層面細胞功能的深入理解。最後，本書涵蓋了用於構建合成細胞的分隔無細胞表達系統，展示了構建具有獨特功能的人工細胞系統的潛力。
鑑於其廣泛性，本書吸引了對生物技術前沿感興趣的學術界、研究人員和專業人士，並與伴隨卷《無細胞大分子合成》一起，兩本書突顯了近幾年無細胞生產系統基礎和應用研究的研究進展，成為該領域中不可或缺的資源。
章節「固定在微粒膜中的多通道電壓門控離子通道的無細胞合成和電生理分析」可在 link.springer.com 上以創用CC 4.0 國際授權開放存取。
作者簡介
Yuan Lu is an Associate Professor at the Department of Chemical Engineering, Tsinghua University, China. After receiving B.S. (2004) and Ph.D. (2009) in chemical engineering from Tsinghua University, China, Dr Lu received postdoctoral training from Johns Hopkins University (2009-2010) and Stanford University (2010-2014), USA. He was a project researcher from 2014 to 2016 at the University of Tokyo, Japan. The central paradigm of Dr Lu's research is using interdisciplinary approaches in engineering, biology, chemistry, physics, and computer science to manipulate biomolecules and complex biological systems. The main focus lies on developing and applying synthetic biology and other cutting-edge technologies to engineer nucleic acids, proteins, pathways, and networks for addressing most daunting challenges in human health.
Michael Jewett is the Walter P. Murphy Professor of Chemical and Biological Engineering and Director of the Center for Synthetic Biology at Northwestern University, USA. Dr Jewett received his PhD in 2005 from Stanford University, USA, completed postdoctoral studies at the Center for Microbial Biotechnology in Denmark and the Harvard University Medical School, and was a guest professor at the Swiss Federal Institute of Technology (ETH Zurich). He is the recipient of the NIH Pathway to Independence Award, David and Lucille Packard Fellowship in Science and Engineering, Camille-Dreyfus Teacher-Scholar Award, and a Finalist for the Blavatnik National Awards for Young Scientists, among others. He is the co-founder of SwiftScale Biologics, Stemloop Inc., Pearl Bio, Induro Therapeutics, and Design Pharmaceuticals. Jewett is a Fellow of AIMBE, AAAS, and NAI. Rather than attempt to balance the tug-of-war that exists between the cell's physiological and evolutionary objectives on one side and the engineer's process objectives on the other, Dr Jewett's group is developing new strategies that widen the aperture of the traditional model of biotechnology. In one direction, the group seeks to create a new paradigm for engineering biocatalytic systems using cell-free biology. The foundational principle is that one can conduct precise, complex biomolecular transformations in crude lysates without using intact cells. This concept represents a significant departure from cell-based processes that rely on microscopic cellular 'reactors, ' and provides an unprecedented and otherwise unattainable freedom of design to modify and control biological systems. Dr Jeweet's groups is also pioneering new directions to repurpose the translation apparatus for synthetic biology. The goal is to monitor, interrogate, and understand the process of translation, and with this knowledge diversify, evolve and repurpose the ribosome and its peripheral machinery into a re-engineered machine to generate non-natural polymers as new classes of sequence-defined, evolvable matter.
作者簡介(中文翻譯)
盧圓是中國清華大學化學工程系的副教授。盧博士於2004年和2009年分別獲得清華大學化學工程學士和博士學位，隨後在美國約翰霍普金斯大學（2009-2010）和斯坦福大學（2010-2014）接受博士後訓練。他於2014年至2016年在日本東京大學擔任專案研究員。盧博士的研究核心範式是利用工程學、生物學、化學、物理學和計算機科學的跨學科方法來操控生物分子和複雜的生物系統。主要重點在於開發和應用合成生物學及其他尖端技術，以工程化核酸、蛋白質、途徑和網絡，解決人類健康面臨的最艱鉅挑戰。
邁克爾·朱維特是美國西北大學化學與生物工程的沃爾特·P·墨菲教授及合成生物學中心的主任。朱維特博士於2005年在美國斯坦福大學獲得博士學位，並在丹麥微生物生物技術中心和哈佛醫學院完成博士後研究，還曾擔任瑞士聯邦理工學院（ETH Zurich）的客座教授。他是美國國立衛生研究院（NIH）獨立之路獎、戴維與露西爾·帕卡德科學與工程獎學金、卡蜜爾·德雷福斯教師學者獎以及布拉瓦特尼克全國青年科學家獎的決賽入圍者等多項獎項的獲得者。他是SwiftScale Biologics、Stemloop Inc.、Pearl Bio、Induro Therapeutics和Design Pharmaceuticals的共同創辦人。朱維特是美國醫學與生物工程學會（AIMBE）、美國科學促進會（AAAS）和國家發明家學院（NAI）的院士。朱維特博士的團隊並不試圖平衡細胞的生理和進化目標與工程師的過程目標之間的拉鋸戰，而是開發新的策略來擴大傳統生物技術模型的視野。在一個方向上，該團隊尋求創造一種新的生物催化系統工程範式，使用無細胞生物學。其基本原則是可以在粗糙的裂解液中進行精確、複雜的生物分子轉化，而無需使用完整的細胞。這一概念代表了從依賴微觀細胞“反應器”的基於細胞的過程的一個重大轉變，並提供了前所未有的設計自由度，以修改和控制生物系統。朱維特博士的團隊還在開創新的方向，以重新利用合成生物學的翻譯裝置。其目標是監測、詢問和理解翻譯過程，並藉此知識多樣化、演化和重新利用核糖體及其周邊機械，將其轉變為重新工程化的機器，以生成非自然聚合物，作為新類別的序列定義、可演化物質。
