Plant Stress Memory: Methods and Protocols
暫譯: 植物壓力記憶:方法與實驗流程

Kumar, Pankaj, Irfan, Mohammad

  • 出版商: Humana Press
  • 出版日期: 2025-10-07
  • 售價: $7,970
  • 貴賓價: 9.5$7,572
  • 語言: 英文
  • 頁數: 216
  • 裝訂: Hardcover - also called cloth, retail trade, or trade
  • ISBN: 1071649809
  • ISBN-13: 9781071649800
  • 相關分類: 其他
  • 海外代購書籍(需單獨結帳)

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商品描述

This detailed volume explores methods and protocols employed to investigate the multifaceted processes involved in plant stress memory. Opening with foundational methods for studying thermomemory in Arabidopsis thaliana, the book continues with techniques for studying memory mechanisms in other plant species, memory of biotic stressors, and the critical role of signaling molecules, such as jasmonic acid and salicylic acid. A key focus of this book is the investigation of DNA methylation and other epigenetic modifications that play a central role in the transmission of stress memory across generations, and epigenetic and transcriptomic approaches plus cutting-edge techniques such as RNA-Seq, CRISPR/Cas9-based gene editing, and proteomic analyses are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Stress Memory: Methods and Protocols serves as an invaluable resource for researchers, students, and practitioners working to unravel the mysteries of plant stress memory and apply this knowledge to address global challenges in agriculture and environmental sustainability.

商品描述(中文翻譯)

這本詳細的專著探討了用於研究植物壓力記憶的多面向過程的方法和協議。書中首先介紹了研究Arabidopsis thaliana熱記憶的基礎方法,接著繼續探討其他植物物種的記憶機制、生物壓力因子的記憶,以及信號分子(如茉莉酸和水楊酸)的關鍵角色。本書的一個重點是研究DNA甲基化及其他在跨代傳遞壓力記憶中扮演核心角色的表觀遺傳修飾,並包含表觀遺傳學和轉錄組學的方法,以及尖端技術如RNA-Seq、基於CRISPR/Cas9的基因編輯和蛋白質組分析。本書是為了成功的Methods in Molecular Biology系列而撰寫,章節包括各自主題的介紹、所需材料和試劑的清單、逐步且易於重複的實驗室協議,以及故障排除和避免已知陷阱的提示。

權威且實用的Plant Stress Memory: Methods and Protocols是研究人員、學生和實踐者在揭開植物壓力記憶之謎並將這些知識應用於解決全球農業和環境可持續性挑戰的寶貴資源。